RealHelix™ qPCR Kit [Green] 은 target DNA에 대한 빠른 실시간 정량 PCR (real-time PCR)을 위한 제품입니다. 제공되는 premix에는 hot-start PCR enzyme, dNTPs, buffer, Mg²⁺, fluorescent green dye 및 안정제(stabilizers)가 2 배 농도로 혼합되어 있어 편리하게 사용할 수 있습니다. Hot-start PCR enzyme을 적용하여 target DNA에 대한 증폭 특이도가 매우 높고(high specificity), 비특이적(non-specific) 증폭 및 primer dimer 생성을 최소화할 수 있습니다.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Green].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the alpha 1-Antitrypsin (AAT) DNA-specific primer set and an intercalating dye. The accuracy of real-time PCR is demonstrated by E=101.7 %, R² value=0.999. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Green] and the previous version (RealHelix™ qPCR Kit [Intercalator type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using a beta-globin gene-specific primer set and an intercalating dye. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months